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Demonstration Experiment on Video

Test for Amylase in Bee Honey

Objective: Enzymatic Hydrolysis of Starch

Peter Keusch




German version



Supermarket products:
bee honey
invert sugar cream (artificial honey)
Dr. Oetker's "Whip it" (whipping cream stabilizer)
Betaisodona® solution  (from Mundipharma, Germany)


"Whip it" is a starch product. It binds the liquid parts of the whipped cream together.
Betaisodona® solution is a non-specific antiseptic agent. It contains povidone-iodine (iodine complex). The desinfecting agent releases iodine, which desactivates viruses.


Apparatus and glass wares:
hotplate
4 beakers 100 mL
glass stirring rod
thermometer


Experimental procedure:

One 100 mL beaker contains 20 mL of bee honey, another contains 20 mL of invert sugar cream. The contents of the beakers are each dissolved in
60 mL of dist. water while stirring. Now 5 mL of the aqueous solution of the cream stabilizer is added to each of the two beakers. The solutions are heated at 40 °C for one hour. Then some drops of the Betaisodona® solution are added to the beakers.


Results:

The solution of the "artificial honey" turns blue. The bee honey solution remains yellowish.




Video clip
(Download RealPlayer .rm file)


Discussion and background:

·   Evidently artificial honey is unable to break down the added starch. The rapid reaction of iodine with starch results in the formation of an iodine starch complex indicated by the instantaneous appearance of a deep violet blue color.











·   Hydrolysis of starch by bee honey results in a negative starch-iodine test. The enzyme amylase available in bee honey breaks down starch. Starch is comprised of two types of polymeric glucose - amylose and amylopectin. Amylose consists of a linear chain of 200 to 1000 glucose residues linked by a-1,4-glycosidic bonds which cause the polymer to form a left-handed helix (Amylose is responsible for the formation of the blue complex in the presence of iodine). On the other hand, amylopectin (contains up to two million glucose units) is a branched polymer. It consists of many amylose chains joined to each other at branch points by a-1,6-glycosidic linkages (1).


Amylose is hydrolyzed to form maltose (2). The enzymatic hydrolysis of amylopectin yields a mixture of branched a-dextrins and maltose.





References:
  Demonstration Experiment on Video   Starch - widely available in many Foods


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