| Demonstration Experiment on Video Objective: Test for Amylase Peter Keusch |
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German version
Experimental procedure: 100 mL of water are heated to boiling in a 250 mL beaker. A suspension of pudding powder (9 g of pudding powder and 2 tablespoons of water) are added while stirring. The mixture, heated for a short time, is divided into two 100 mL beakers. 2 g of amylase containing washing powder are added to one of the two beakers. The samples are allowed to cool down to room temperature.
Discussion and background: Pudding powder is a specificially treated potato starch or a mixture of native starches coming from different species of plants. When pudding powder is dispersed in water and heated above 60 °C, the starch granules absorb water, swell, disrupt and a gel is formed during cooling. Amylase digests starch into smaller disaccharide units, eventually converting them into monosaccharides such as glucose. The swelled starch polymers of the pudding are hydrolyzed; therefore the pudding treated with amylase does not become firm while cooling. Because of its properties, starch acts as a binding agent and as an extender and carrier in food industry.
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