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Demonstration Experiment on Video

Amylase makes Detergents more effective

Objective: Test for Amylase

Peter Keusch




German version



Supermarket products:
Washing powder with amylase  rei F3,  ingredients: 5 - 15 % anionic tensides, < 5 % non-ionic tensides, polycarboxylates, soap, phosphonates, enzymes (amylase, cellulase), citrate, perfume
Pudding powder,  Dr. Oetker Pudding Strawberry,  ingredients:  starch, flavoring agent, table salt, dyes (Cochenille Red A, Azorubine)

Apparatus and glass wares:
hotplate
beaker 250 mL
3 beakers 100 mL
snap-cap vial 20 mL
2 watch glasses d = 8 cm
glass stirring rod


Experimental procedure:


100 mL of water are heated to boiling in a 250 mL beaker. A suspension of pudding powder (9 g of pudding powder and 2 tablespoons of water) are added while stirring. The mixture, heated for a short time, is divided into two 100 mL beakers. 2 g of amylase containing washing powder are added to one of the two beakers. The samples are allowed to cool down to room temperature.


Results:

The pudding treated with the washing powder is liquid. The other untreated sample has become firm after cooling to room temperature.


Video clip (Download RealPlayer .rm file)


Discussion and background:

Pudding powder is a specificially treated potato starch or a mixture of native starches coming from different species of plants. When pudding powder is dispersed in water and heated above 60 °C, the starch granules absorb water, swell, disrupt and a gel is formed during cooling.

Amylase digests starch into smaller disaccharide units, eventually converting them into monosaccharides such as glucose. The swelled starch polymers of the pudding are hydrolyzed; therefore the pudding treated with amylase does not become firm while cooling.

Because of its properties, starch acts as a binding agent and as an extender and carrier in food industry.







Amylase facilitates the removal of starch containing stains, e.g. from pasta, potato, gravy, chocolate and baby food.


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